RESUMO
Viruses isolated during 1979 and 1980 from patients with polyarthritis in New Caledonia and Wallis and Futuna Islands have been found to be more closely related to Ross River virus than any other regional Alphavirus. On the basis of virulence in suckling mice the majority of these isolates were found to be more closely related to the NB5092 strain of Ross River virus than to the prototype T48 strain.
Assuntos
Alphavirus/isolamento & purificação , Artrite Infecciosa/microbiologia , Vírus do Rio Ross/isolamento & purificação , Infecções por Togaviridae/microbiologia , Animais , Austrália , Eletroforese em Gel de Poliacrilamida , Humanos , Camundongos , Nova Caledônia , Polinésia , Ensaio de Placa ViralRESUMO
An ELISA method for the detection of IgM antibody to Ross River virus in the serum of patients with suspected epidemic polyarthritis has been developed. None of the reagents is infectious or toxic and the use of purified virus minimizes the risk of non-specific reactions to non-viral products. In a positive reaction the ELISA substrate changes colour from yellow to purple, enabling serum antibody titres to be read by eye.
